| 1. | Preliminary detection of polymorphisms of expressed sequence tag in pinus massoniana
马尾松表达序列标签多态性初步分析 |
| 2. | Expressed sequence tag
表达序列标签 |
| 3. | In addition , more than 30 differential expressed sequence tags ( est ) have been isolated by mrna differential display
我们还利用mrna差异显示技术分离到了近30个在mnng处理后表达改变的表达序列标签（ expressedsequenceag ， est ） 。 |
| 4. | We have identified a nucleotide sequence from ests ( expressed sequence tags ) acquired form a cdna library of thellungiella halophila treated with 200mm / nacl by the large - scale partial sequencing of randomly selected cdna clones
通过对小盐芥的cdna文库大量测序，在1000个est序列中获得14个编码th - nsltp的序列，这表明它是一个中等丰度的基因。 |
| 5. | Bioinformatics methods are used to analyze and manage expressed sequence tags ( ests ) which are generated by large - scale sequencing of a cdna library from cephalothorax of a wild , female , adult chinese shrimp ( fenneropenaeus chinensis )
本文运用生物信息学的方法，分析及管理由雌性成年中国对虾头胸部cdna文库经部分测序产生的10446条ests 。 |
| 6. | Gene ( tscoxi ) , structure protein gene ( tsdcn , tsmmip , tsb - actiri ) , transcriptional factor gene ( tshmg1 , tsdap5 ) . the cdna library can be used to provide expressed sequence tags ( ests ) . the stage - specific cdna library will be a useful resource for the study of gene structure , expression and regulatory during the early process of embroygensis of trionyx sinensis
Blast分析表明， 12个序列代表5种类型的基因:核糖体蛋白基因( tsrpl4 ， tsrp丈6 ，招天尸乙26 ) 、组织特异性表达的基因(钻尸乃从招月叨火d ) 、线粒体基因( tsc “叨、结构蛋白基因伽dc从括人从石， ， ts刀一ctin ) 、编码转录因子的基因(招月沏吟1 ， tsda尸5 ) 。 |
| 7. | Expressed sequence tag ( est ) analysis was carried out to study the molecular mechanism of salt tolerance for polygonum sibiricum laxm . . a cdna library was constructed from polygonum sibiricum laxm . treated by salt using stratagene cdna synthesis & gigapack iii gold cloning kit
为了研究西伯利亚蓼耐盐的分子机理，使用stratagene的cdna合成和gigapack gold包装试剂盒构建了盐( nahco _ 3 )胁迫下西伯利亚蓼的cdna文库，随机选取重组克隆测序，用表达序列标签( est )方法研究盐胁迫下西伯利亚蓼基因的表达。 |
| 8. | Previously , we have constructed a 400mmol / l nacl - treated cdna library of s . salsa and acquired 1000 ests ( expressed sequence tags ) by the large - scale partial sequencing of randomly selected cdna clones . in this research , we isolated a cdna that maybe encode an aquaporin ( s . salsa plasma membrane intrinsic protein , sspip , bf023789 ) located in plasma membrane of s . salsa . then we analyzed the sequence characterizations , genomic structures and transcriptional levels under salinity stress
本实验室构建了400mmol lnacl处理的盐地碱蓬( s . salsa )地上组织的cdna文库，随机挑取单克隆进行测序，获得了可能编码盐地碱蓬质膜水通道蛋白( s . salsaplasmamembraneintrinsicprotein ， sspip )的cdna ，其序列号为bf023789 。 |
| 9. | A cdna library of grass carp ( ctenopharyngodon idellus ) leukocytes stimulated with virus was constructed in order to clone and analyze the immune genes which are induced and expressed by virus infection in the immune system of this fish . initial studies on rapid identification of fish ' s new genes by expressed sequence tag analysis were also undertook in this paper
为了克隆草鱼免疫相关基因，我们构建了草鱼白细胞cdna文库，同时对利用生物信息资源快速鉴定鱼类新的功能基因的方法进行了初步探讨，为今后深入开展鱼类功能基因研究奠定了良好的基础。 |
| 10. | In summary , we applied rd - pcr technique to the isolation of cdna fragments , which , compared with the conventional cloning procedure , is more speedy and higher throughput . the rd - pcr cdna fragments were constructed into a s . cerevisiae est ( expressed sequence tags ) library , which lay the foundation of cdna probe preparation and preparation of cdna microarray
综上所述，本研究采用rd ? pcr技术，探索了利用该技术快速分离大量cdna片段的具体过程，并建立了酵母cdna文库及酵母cdna探针文库，为dna芯片的制备打好基础。 |
